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resource source identifier rat monoclonal anti cd206  (Bio-Rad)


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    Bio-Rad resource source identifier rat monoclonal anti cd206
    Resource Source Identifier Rat Monoclonal Anti Cd206, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resource source identifier rat monoclonal anti cd206/product/Bio-Rad
    Average 96 stars, based on 619 article reviews
    resource source identifier rat monoclonal anti cd206 - by Bioz Stars, 2026-03
    96/100 stars

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    Dot plots of Siglec gene expression levels at single cell resolution in prostate tumours using previously published single cell RNA-sequencing data from ( A ) primary prostate tumours and ( B ) prostate cancer bone metastatic tissues . ( C ) Dual immunofluorescence analysis of Siglec receptors and immune cell markers in independent prostate cancer bone metastasis tissue samples. This analysis was also carried out for primary prostate tissue (Supplementary Figure 4). Siglec-7, -9, -10 and -15 are co-expressed with CD14 (a myeloid marker) and <t>CD206</t> (an M2 macrophage marker) in bone metastatic tumours. Furthermore, we reveal Siglec-3 and -15 are expressed with cathepsin K (an osteoclast marker). Scale bar is 20□µm.
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    Dot plots of Siglec gene expression levels at single cell resolution in prostate tumours using previously published single cell RNA-sequencing data from ( A ) primary prostate tumours and ( B ) prostate cancer bone metastatic tissues . ( C ) Dual immunofluorescence analysis of Siglec receptors and immune cell markers in independent prostate cancer bone metastasis tissue samples. This analysis was also carried out for primary prostate tissue (Supplementary Figure 4). Siglec-7, -9, -10 and -15 are co-expressed with CD14 (a myeloid marker) and <t>CD206</t> (an M2 macrophage marker) in bone metastatic tumours. Furthermore, we reveal Siglec-3 and -15 are expressed with cathepsin K (an osteoclast marker). Scale bar is 20□µm.
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    Dot plots of Siglec gene expression levels at single cell resolution in prostate tumours using previously published single cell RNA-sequencing data from ( A ) primary prostate tumours and ( B ) prostate cancer bone metastatic tissues . ( C ) Dual immunofluorescence analysis of Siglec receptors and immune cell markers in independent prostate cancer bone metastasis tissue samples. This analysis was also carried out for primary prostate tissue (Supplementary Figure 4). Siglec-7, -9, -10 and -15 are co-expressed with CD14 (a myeloid marker) and CD206 (an M2 macrophage marker) in bone metastatic tumours. Furthermore, we reveal Siglec-3 and -15 are expressed with cathepsin K (an osteoclast marker). Scale bar is 20□µm.

    Journal: bioRxiv

    Article Title: Siglec-engaging immunosuppressive sialoglycans are upregulated in prostate cancer and are targetable to suppress bone metastasis

    doi: 10.1101/2025.11.12.687981

    Figure Lengend Snippet: Dot plots of Siglec gene expression levels at single cell resolution in prostate tumours using previously published single cell RNA-sequencing data from ( A ) primary prostate tumours and ( B ) prostate cancer bone metastatic tissues . ( C ) Dual immunofluorescence analysis of Siglec receptors and immune cell markers in independent prostate cancer bone metastasis tissue samples. This analysis was also carried out for primary prostate tissue (Supplementary Figure 4). Siglec-7, -9, -10 and -15 are co-expressed with CD14 (a myeloid marker) and CD206 (an M2 macrophage marker) in bone metastatic tumours. Furthermore, we reveal Siglec-3 and -15 are expressed with cathepsin K (an osteoclast marker). Scale bar is 20□µm.

    Article Snippet: Tissues were then blocked with 1X CFB for 1 hr at room temperature, following overnight incubation in the dark at 4 °C in 1:400 CD22 Monoclonal Antibody (Proteintech, 66103-1-Ig), 1:200 Anti-CD33 Antibody [WM53] (Abcam, ab252263), 1:200 Siglec-7/CD328 Polyclonal Antibody (Proteintech, 13939-1-AP), 1:200 Siglec-9 Polyclonal Antibody (Proteintech, 13377-1-AP), 1:100 Siglec-10 Polyclonal Antibody (Invitrogen, PA5-55501), 1:100 Siglec-15 Polyclonal Antibody (Abcam, ab198684), 1:50 Mouse Siglec-E Antibody (R&D Systems, AF5806), 1:200 CD14 Rabbit Polyclonal Antibody (Proteintech, 17000-1-AP), 1:200 CD14 Mouse Monoclonal Antibody (Proteintech, 60253-1-Ig), 1:500 AMACR/p504S Rabbit Polyclonal Antibody (Proteintech, 15918-1-AP), 1:200 AMACR Mouse Monoclonal Antibody [UMAB68] (UltraMAB, UM870012), 1:200 CD206 Rabbit Polyclonal Antibody (Proteintech, 18704-1-AP), 1:200 CD206 Mouse Monoclonal Antibody (Proteintech, 60143-1-Ig), 1:200 Cathepsin K Rabbit Polyclonal Antibody (Proteintech, 11239-1-AP), 1:200 Cathepsin K Mouse Monoclonal Antibody [E-7] (Santa Cruz, sc-48353), 1:200 CD20 Rabbit Polyclonal Antibody (Proteintech, 24828-1-AP) or 1:100 Pan Cytokeratin Monoclonal Antibody (AE1/AE3), Alexa FluorTM 488-conjugated (Invitrogen 53-9003-80).

    Techniques: Gene Expression, RNA Sequencing, Immunofluorescence, Marker